PPARD Antibody

• Background :

  PPARD is a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR delta and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation.

• NCBI Gene ID :

  5467

• Swiss Prot :

  Q03181

• Accession Number :

  NP_006229

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PPARD.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Transcription, Cancer

• Purification :

  Antibody is purified by protein A chromatography method.

• Positive Control :

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PPARD antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  49 kDa

• Applications Notes :

  PPARD antibody can be used for detection of PPARD by ELISA at 1:1562500. PPARD antibody can be used for detection of PPARD by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  PPARD

• NCBI Official Name :

  Peroxisome proliferator-activated receptor delta

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Barish, G.D., et al., (2006) J. Clin. Invest. 116 (3), 590-597.

• Other Product Names :

  PPARD, FAAR, NUC1, NUCI, NR1C2, NUCII, PPARB

• Tested Applications :

  ELISA, WB

• Protein ID :

  5453940

• Physical Properties :

  Liquid