NFIX Antibody

• Background:

  Nuclear factor I (NFI) proteins constitute a family of sequence-specific transcription factors whose functional diversity is generated through transcription from four different genes (NFI-A, NFI-B, NFI-C, and NFI-X), alternative RNA splicing, and protein heterodimerization. NFI-X has divergent functions after binding in promoter or enhancer position. This property, combined with the differential expression of NFI-X, can achieve cell-type specificity of NFI dependent promoters and enhancers.

• NCBI Gene ID:

  4784

• Swiss Prot:

  Q14938-3

• Accession Number:

  NP_002492

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFIX.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cell Cycle

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NFIX antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  49 kDa

• Applications Notes:

  NFIX antibody can be used for detection of NFIX by ELISA at 1:312500. NFIX antibody can be used for detection of NFIX by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NFIX

• NCBI Official Name:

  Nuclear factor I/X (CCAAT-binding transcription factor)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Norquay, L.D., et al., (2003) Mol. Endocrinol. 17 (6), 1027-1038.

• Other Product Names:

  NFIX, NF1A, MRSHSS, SOTOS2

• Tested Applications:

  ELISA, WB

• Protein ID:

  56549649

• Physical Properties:

  Liquid