AIRE Antibody

• Background:

  AIRE is a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD) . Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) .

• NCBI Gene ID:

  326

• Swiss Prot:

  O43918

• Accession Number:

  NP_000374

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AIRE.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Signal Transduction, Immunology

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store AIRE antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  58 kDa

• Applications Notes:

  AIRE antibody can be used for detection of AIRE by ELISA at 1:1562500. AIRE antibody can be used for detection of AIRE by western blot at 5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  AIRE

• NCBI Official Name:

  Autoimmune regulator

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Tao, Y., et al., (2006) Mol. Immunol. 43 (4), 335-345.

• Other Product Names:

  AIRE, APS1, APSI, PGA1, AIRE1, APECED

• Tested Applications:

  ELISA, WB

• Protein ID:

  4557291

• Physical Properties:

  Liquid