SIRT1 Antibody

• Background:

  SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family.

• NCBI Gene ID:

  23411

• Swiss Prot:

  Q96EB6

• Accession Number:

  NP_036370

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIRT1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SIRT1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  82 kDa

• Applications Notes:

  SIRT1 antibody can be used for detection of SIRT1 by ELISA at 1:62500. SIRT1 antibody can be used for detection of SIRT1 by western blot at 5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  SIRT1

• NCBI Official Name:

  Sirtuin 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Solomon, J.M., et al., (2006) Mol. Cell. Biol. 26 (1), 28-38.

• Other Product Names:

  SIRT1, SIR2L1

• Tested Applications:

  ELISA, WB

• Protein ID:

  7657575

• Physical Properties:

  Liquid