SirT1 (phospho Ser47) Antibody

• Background:

  NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively.

• NCBI Gene ID:

  23411

• Swiss Prot:

  Q96EB6

• Accession Number:

  NP_001135970.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  SirT1 (phospho Ser47) antibody was raised against a peptide sequence around phosphorylation site of serine 47 (E-R-S (p) -P-G) derived from Human SirT1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  110 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous level of SirT1 only when phosphorylated at serine 47.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  SIRT1

• NCBI Official Name:

  Sirtuin 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Gao Z, et al. (2011) J Biol Chem 286, 22227-34. Zschoernig B, Mahlknecht U (2009) Biochem Biophys Res Commun 381, 372-7. Nasrin N, et al. (2009) PLoS One 4, e8414.

• Other Product Names:

  HSIR2, hSIRT1, NAD-dependent deacetylase sirtuin 1, SIR1, SIR2L1

• Tested Applications:

  WB

• Protein ID:

  7657575

• Physical Properties:

  Liquid