MTF1 Antibody

• Background:

  The zinc finger transcription factor MTF-1 (metal-responsive transcription factor-1) is conserved from insects to vertebrates. The major role of MTF-1 in both organisms is to control the transcription of genes involved in the homeostasis and detoxification of heavy metal ions such as Cu2+, Zn2+ and Cd2+. In mammals, MTF-1 serves at least two additional roles. First, targeted disruption of the MTF-1 gene results in death at embryonic day 14 due to liver degeneration, revealing a stage-specific developmental role. Second, under hypoxic-anoxic stress, MTF-1 helps to activate the transcription of the gene placental growth factor (PIGF), an angiogenic protein.

• NCBI Gene ID:

  4520

• Swiss Prot:

  Q14872

• Accession Number:

  NP_005946

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MTF1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MTF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  81 kDa

• Applications Notes:

  MTF1 antibody can be used for detection of MTF1 by ELISA at 1:2500. MTF1 antibody can be used for detection of MTF1 by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MTF1

• NCBI Official Name:

  Metal-regulatory transcription factor 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chen, X., et al., (2004) J. Biol. Chem. 279 (6), 4515-4522.

• Other Product Names:

  MTF1, ZRF, MTF-1

• Tested Applications:

  ELISA, WB

• Protein ID:

  112790163

• Physical Properties:

  Liquid