SPDEF Antibody

• Background:

  PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA expression.PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.

• NCBI Gene ID:

  25803

• Swiss Prot:

  O95238

• Accession Number:

  NP_036523

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPDEF.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1219 - MCF7 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SPDEF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  38 kDa

• Applications Notes:

  SPDEF antibody can be used for detection of SPDEF by ELISA at 1:12500. SPDEF antibody can be used for detection of SPDEF by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  SPDEF

• NCBI Official Name:

  SAM pointed domain containing ETS transcription factor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Fowler, M., (2006) J. Cell. Biochem. 97 (1), 1-17.

• Other Product Names:

  SPDEF, PDEF, RP11-375E1__A.3, bA375E1.3

• Tested Applications:

  ELISA, WB

• Protein ID:

  6912580

• Physical Properties:

  Liquid