E2F4 Antibody

• Background :

  The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins. It is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It also plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.

• NCBI Gene ID :

  1874

• Swiss Prot :

  Q16254

• Accession Number :

  NP_001941

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Dog

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F4.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Transcription, Cell Cycle, Cancer

• Purification :

  Antibody is purified by peptide affinity chromatography method.

• Positive Control :

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store E2F4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  44 kDa

• Applications Notes :

  E2F4 antibody can be used for detection of E2F4 by ELISA at 1:312500. E2F4 antibody can be used for detection of E2F4 by western blot at 2.0/mL.

• Symbol :

  E2F4

• NCBI Official Name :

  E2F transcription factor 4, p107/p130-binding

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  DuPree, E.L., et al., (2004) Cancer Res. 64 (13), 4390-4393.

• Other Product Names :

  E2F4, E2F-4

• Tested Applications :

  ELISA, WB

• Protein ID :

  12669915

• Physical Properties :

  Liquid