E2F4 Antibody

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins. It is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It also plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.

1874

Q16254

NP_001941

Human, Mouse, Dog

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F4.

Unconjugated

Primary Antibodies

Transcription, Cell Cycle, Cancer

Antibody is purified by peptide affinity chromatography method.

Cat. No. 1211 - HepG2 Cell Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store E2F4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

44 kDa

E2F4 antibody can be used for detection of E2F4 by ELISA at 1:312500. E2F4 antibody can be used for detection of E2F4 by western blot at 2.0/mL.

E2F4

E2F transcription factor 4, p107/p130-binding

Homo sapiens

DuPree, E.L., et al., (2004) Cancer Res. 64 (13), 4390-4393.

E2F4, E2F-4

ELISA, WB

12669915