TAF1A Antibody

• Background:

  Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, also known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.

• NCBI Gene ID:

  9015

• Swiss Prot:

  Q15573

• Accession Number:

  NP_005672

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF1A.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by Protein A/G affinity chromatography method.

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TAF1A antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  53 kDa

• Applications Notes:

  TAF1A antibody can be used for detection of TAF1A by ELISA at 1:15000. TAF1A antibody can be used for detection of TAF1A by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  TAF1A

• NCBI Official Name:

  TATA box binding protein (TBP) -associated factor, RNA polymerase I, A, 48kDa

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Di Pietro, C., et al., (2000) Cytogenet. Cell Genet. 89 (1-2) 133-136..

• Other Product Names:

  TAF1A, SL1, RAFI48, TAFI48, MGC:17061

• Tested Applications:

  ELISA, WB

• Protein ID:

  5032143

• Physical Properties:

  Liquid