LEF1 Antibody

• Background :

  Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1) . Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905) .

• NCBI Gene ID :

  51176

• Swiss Prot :

  Q9UJU2

• Accession Number :

  NP_057353

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LEF1.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Transcription, Cancer, Signal Transduction

• Purification :

  Antibody is purified by peptide affinity chromatography method.

• Positive Control :

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store LEF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  44 kDa

• Applications Notes :

  LEF1 antibody can be used for detection of LEF1 by ELISA at 1:312500. LEF1 antibody can be used for detection of LEF1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  LEF1

• NCBI Official Name :

  Lymphoid enhancer-binding factor 1

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Petropoulos, K., (2008) J. Exp. Med. 205 (3), 515-522.

• Other Product Names :

  LEF1, DKFZp586H0919, TCF1ALPHA, LEF-1, TCF10, TCF7L3

• Tested Applications :

  ELISA, WB

• Protein ID :

  7705917

• Physical Properties :

  Liquid