UCHL3 Antibody

• Background:

  UCHL3 is an ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8.

• NCBI Gene ID:

  7347

• Swiss Prot:

  P15374

• Accession Number:

  NP_005993

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UCHL3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1219 - MCF7 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store UCHL3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  25 kDa

• Applications Notes:

  UCHL3 antibody can be used for detection of UCHL3 by ELISA at 1:1562500. UCHL3 antibody can be used for detection of UCHL3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  UCHL3

• NCBI Official Name:

  Ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lim, J., (2006) Cell 125 (4), 801-814.

• Other Product Names:

  UCHL3, UCH-L3

• Tested Applications:

  ELISA, WB

• Protein ID:

  5174741

• Physical Properties:

  Liquid