KRAS Antibody

• Background :

  KRAS is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

• NCBI Gene ID :

  3845

• Swiss Prot :

  P01111

• Accession Number :

  NP_203524

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat, Drosophila

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KRAS.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cell Cycle, Cancer, Signal Transduction, Immunology

• Purification :

  Antibody is purified by peptide affinity chromatography method.

• Positive Control :

  Cat. No. XBL-10407 - Fetal Heart Tissue Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store KRAS antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  22 kDa

• Applications Notes :

  KRAS antibody can be used for detection of KRAS by ELISA at 1:62500. KRAS antibody can be used for detection of KRAS by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  KRAS

• NCBI Official Name :

  Kirsten rat sarcoma viral oncogene homolog

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Turbiner, J., (er) Mod. Pathol. (2008) In press.

• Other Product Names :

  KRAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2, NS, CFC2

• Tested Applications :

  ELISA, WB

• Protein ID :

  15718763

• Physical Properties :

  Liquid