ENPP6 Antibody

• Background:

  ENPP6 is a choline-specific glycerophosphodiester phosphodiesterase. ENPP6 hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate. ENPP6 hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. ENPP6 has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently.

• NCBI Gene ID:

  133121

• Swiss Prot:

  Q6UWR7

• Accession Number:

  NP_699174

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ENPP6.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  721_B Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ENPP6 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  50 kDa

• Applications Notes:

  ENPP6 antibody can be used for detection of ENPP6 by ELISA at 1:1562500. ENPP6 antibody can be used for detection of ENPP6 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  ENPP6

• NCBI Official Name:

  Ectonucleotide pyrophosphatase/phosphodiesterase 6

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sakagami, H., (2005) J. Biol. Chem. 280 (24), 23084-23093.

• Other Product Names:

  ENPP6, MGC33971, NPP6

• Tested Applications:

  ELISA, WB

• Protein ID:

  23503267

• Physical Properties:

  Liquid