SGMS2 Antibody

• Background:

  SGMS2 is a bidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. SGMS2 directly and specifically recognizes the choline head group on the substrate. SGMS2 also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. SGMS2 does not function strictly as a SM synthase. SGMS2 is required for cell growth.Sphingomyelin (SM) is a major component of plasma membranes. It is preferentially concentrated in the outer leaflet and has a role in the formation of lipid rafts. SM synthases (EC 2.7.8.27), such as SGMS2, produce SM in the lumen of the Golgi and on the cell surface through the transfer of phosphocholine from phosphatidylcholine onto ceramide, yielding diacylglycerol as a side product (Huitema et al., 2004 [PubMed 14685263]) .

• NCBI Gene ID:

  166929

• Swiss Prot:

  Q8NHU3

• Accession Number:

  NP_689834

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SGMS2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Membrane

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  293T Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SGMS2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  42 kDa

• Applications Notes:

  SGMS2 antibody can be used for detection of SGMS2 by ELISA at 1:1562500. SGMS2 antibody can be used for detection of SGMS2 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  SGMS2

• NCBI Official Name:

  Sphingomyelin synthase 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ding, T., (2008) J. Lipid Res. 49 (2), 376-385.

• Other Product Names:

  SGMS2, MGC26963, SMS2

• Tested Applications:

  ELISA, WB

• Protein ID:

  22749271

• Physical Properties:

  Liquid