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NSUN2 Antibody

CAT: 0223-26-350Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-26-350Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA) .Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA) (Brzezicha et al., 2006 [PubMed 17071714]) .
NCBI Gene ID
54888
Swiss Prot
Q08J23
Accession Number
NP_060225
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NSUN2.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1201 - HeLa Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NSUN2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
86 kDa
Applications Notes
NSUN2 antibody can be used for detection of NSUN2 by ELISA at 1:62500. NSUN2 antibody can be used for detection of NSUN2 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
NSUN2
NCBI Official Name
NOP2/Sun RNA methyltransferase family, member 2
NCBI Organism
Homo sapiens
Background Reference 01
Huttenhofer, A., (2007) Mol. Biol. Cell 18 (3), 1107-1117.
Other Product Names
NSUN2, FLJ20303, MISU, SAKI, TRM4, MRT5
Tested Applications
ELISA, WB
Protein ID
39995082
Physical Properties
Liquid

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