KIRREL Antibody

• Background:

  KIRREL (NEPH1) is a member of the nephrin-like protein family, which includes NEPH2 and NEPH3. The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration. NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762) . The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]) .

• NCBI Gene ID:

  55243

• Swiss Prot:

  Q5W0F9

• Accession Number:

  NP_060710

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KIRREL.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Membrane

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store KIRREL antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  66 kDa

• Applications Notes:

  KIRREL antibody can be used for detection of KIRREL by ELISA at 1:312500. KIRREL antibody can be used for detection of KIRREL by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  KIRREL

• NCBI Official Name:

  Kin of IRRE like (Drosophila)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Harita, Y., (2008) J. Biol. Chem. 283 (14), 9177-9186.

• Other Product Names:

  KIRREL, FLJ10845, MGC129542, MGC129543, NEPH1

• Tested Applications:

  ELISA, WB

• Protein ID:

  166295173

• Physical Properties:

  Liquid