ATP6V0A2 Antibody

• Background:

  The multisubunit vacuolar-type proton pump (H (+) -ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H (+) -ATPases are comprised of a peripheral V (1) domain and an integral membrane V (0) domain; ATP6V0A2 is a component of the V (0) domain.The multisubunit vacuolar-type proton pump (H (+) -ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H (+) -ATPases are comprised of a peripheral V (1) domain and an integral membrane V (0) domain; ATP6V0A2 is a component of the V (0) domain (Smith et al., 2003 [PubMed 14580332]) .

• NCBI Gene ID:

  23545

• Swiss Prot:

  Q9Y487

• Accession Number:

  NP_036595

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP6V0A2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Membrane, Signal Transduction, Immunology

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ATP6V0A2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  98 kDa

• Applications Notes:

  ATP6V0A2 antibody can be used for detection of ATP6V0A2 by ELISA at 1:312500. ATP6V0A2 antibody can be used for detection of ATP6V0A2 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  ATP6V0A2

• NCBI Official Name:

  ATPase, H+ transporting, lysosomal V0 subunit a2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kornak, U., (2008) Nat. Genet. 40 (1), 32-34.

• Other Product Names:

  ATP6V0A2, ATP6N1D, ATP6a2, J6B7, Stv1, TJ6, TJ6M, TJ6s, Vph1, a2, A2, RTF, WSS, ARCL, STV1, TJ6S, VPH1, ARCL2A, ATP6A2

• Tested Applications:

  ELISA, WB

• Protein ID:

  42741679

• Physical Properties:

  Liquid