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PAPOLG Antibody

CAT: 0223-25-659Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-25-659Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
PAPOLG is a member of the poly (A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly (A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor) /AAUAAA-dependent polyadenylation activity.This gene encodes a member of the poly (A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly (A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor) /AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14.
NCBI Gene ID
64895
Swiss Prot
Q9BWT3
Accession Number
NP_075045
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAPOLG.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1201 - HeLa Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PAPOLG antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
83 kDa
Applications Notes
PAPOLG antibody can be used for detection of PAPOLG by ELISA at 1:1562500. PAPOLG antibody can be used for detection of PAPOLG by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
PAPOLG
NCBI Official Name
Poly (A) polymerase gamma
NCBI Organism
Homo sapiens
Background Reference 01
Beausoleil, S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135.
Other Product Names
PAPOLG, FLJ11805, FLJ12972, FLJ13482, FLJ14187, MGC133307, MGC133308, PAP2, PAPG
Tested Applications
ELISA, WB
Protein ID
16306568
Physical Properties
Liquid

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