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RFX1 Antibody

CAT: 0223-25-416Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-25-416Size:100 µL
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Background
RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX1 is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
NCBI Gene ID
5989
Swiss Prot
P22670
Accession Number
NP_002909
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFX1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Signal Transduction, Immunology
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Tranfected 293T Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store RFX1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
105 kDa
Applications Notes
RFX1 antibody can be used for detection of RFX1 by ELISA at 1:62500. RFX1 antibody can be used for detection of RFX1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
RFX1
NCBI Official Name
Regulatory factor X, 1 (influences HLA class II expression)
NCBI Organism
Homo sapiens
Background Reference 01
Wang, K.R., (2007) J. Biol. Chem. 282 (36), 26167-26177.
Other Product Names
RFX1, EF-C, EFC, RFX
Tested Applications
ELISA, WB
Protein ID
238859557
Physical Properties
Liquid

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