NR1I2 Antibody

• Background:

  NR1I2 belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. NR1I2 contains a zinc finger domain.NR1I2 is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. NR1I2 belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized.

• NCBI Gene ID:

  8856

• Swiss Prot:

  O75469

• Accession Number:

  NP_003880

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR1I2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer, Signal Transduction

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NR1I2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  50 kDa

• Applications Notes:

  NR1I2 antibody can be used for detection of NR1I2 by ELISA at 1:1562500. NR1I2 antibody can be used for detection of NR1I2 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NR1I2

• NCBI Official Name:

  Nuclear receptor subfamily 1, group I, member 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hesselink, D.A., (2008) Pharmacogenomics 9 (6), 783-789.

• Other Product Names:

  NR1I2, BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR

• Tested Applications:

  ELISA, WB

• Protein ID:

  34398348

• Physical Properties:

  Liquid