SSX4 Antibody

• Background:

  SSX4 belongs to the SSX family. It contains 1 KRAB-related domain.SSX4 could act as a modulator of transcription. The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t (X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma, X breakpoint 4, SSX4 and SSX4B, in tail-to-tail orientation. This gene, SSX4, represents the more telomeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene.

• NCBI Gene ID:

  6759

• Swiss Prot:

  O60224

• Accession Number:

  NP_005627

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SSX4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SSX4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  22 kDa

• Applications Notes:

  SSX4 antibody can be used for detection of SSX4 by ELISA at 1:312500. SSX4 antibody can be used for detection of SSX4 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  SSX4

• NCBI Official Name:

  Synovial sarcoma, X breakpoint 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ayyoub, M., (2005) J. Immunol. 174 (8), 5092-5099.

• Other Product Names:

  SSX4, MGC119056, MGC12411, CT5.4

• Tested Applications:

  ELISA, WB

• Protein ID:

  5032123

• Physical Properties:

  Liquid