HSBP1 Antibody

• Background :

  The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1 (HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response.The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1 (HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response.

• NCBI Gene ID :

  3281

• Swiss Prot :

  O75506

• Accession Number :

  NP_001528

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSBP1.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Transcription

• Purification :

  Antibody is purified by peptide affinity chromatography method.

• Positive Control :

  Cat. No. XBL-10123 - Fetal Brain Tissue Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store HSBP1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  8 kDa

• Applications Notes :

  HSBP1 antibody can be used for detection of HSBP1 by ELISA at 1:312500. HSBP1 antibody can be used for detection of HSBP1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  HSBP1

• NCBI Official Name :

  Heat shock factor binding protein 1

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Tai, L.J., (2002) J. Biol. Chem. 277 (1), 735-745.

• Other Product Names :

  HSBP1, DKFZp686D1664, DKFZp686O24200, NPC-A-13

• Tested Applications :

  ELISA, WB

• Protein ID :

  4557647

• Physical Properties :

  Liquid