CYB5R3 Antibody

• Background:

  This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias.

• NCBI Gene ID:

  1727

• Swiss Prot:

  P00387

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 125-334 of human CYB5R3 (NP_001165131.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  Affinity purification

• Positive Control:

  HepG2

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 34-36kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:500 - 1:2000

• Symbol:

  CYB5R3

• Positive Control 2:

  HeLa

• Positive Control 3:

  HT-1080

• Positive Control 4:

  NIH/3T3

• Positive Control 5:

  Mouse kidney

• Positive Control 6:

  Mouse brain

• NCBI Official Name:

  Cytochrome b5 reductase 3

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  OTTHUMP00000198665, OTTHUMP00000198574, NADH-cytochrome b5 reductase 3 soluble form, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3, DIA1, B5R, cytochrome b5 reductase 3, CYB5R3

• Tested Applications:

  WB

• Physical Properties:

  Liquid