MECP2 Antibody

• Background:

  DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD) . Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Alternative splicing results in multiple transcript variants encoding different isoforms.

• NCBI Gene ID:

  4204

• Swiss Prot:

  P51608

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MECP2 (NP_004983.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  Affinity purification

• Positive Control:

  MCF7

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 52kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:500 - 1:2000

• Symbol:

  MECP2

• Positive Control 2:

  SW620

• Positive Control 3:

  HT-29

• Positive Control 4:

  Jurkat

• Positive Control 5:

  Mouse spleen

• Positive Control 6:

  Mouse lung

• NCBI Official Name:

  Methyl CpG binding protein 2 (Rett syndrome)

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  MECP2, AUTSX3, DKFZp686A24160, MRX16, MRX79, PPMX, RTS, RTT, RS, MRXSL, MRXS13

• Tested Applications:

  WB

• Physical Properties:

  Liquid