CLOCK Antibody

• Background:

  The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants.

• NCBI Gene ID:

  9575

• Swiss Prot:

  O15516

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 577-846 of human CLOCK (NP_004889.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience, Signal Transduction, Transcription

• Purification:

  Affinity purification

• Positive Control:

  MCF7

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 70kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:500 - 1:2000

• Symbol:

  CLOCK

• Positive Control 2:

  BT-474

• Positive Control 3:

  NCI-H460

• Positive Control 4:

  HepG2

• Positive Control 5:

  BxPC-3

• Positive Control 6:

  Mouse lung

• NCBI Official Name:

  Clock circadian regulator

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  CLOCK, clock homolog (mouse), KAT13D, bHLHe8, circadian locomoter output cycles kaput protein, circadian locomoter output cycles protein kaput, class E basic helix-loop-helix protein 8

• Tested Applications:

  WB

• Physical Properties:

  Liquid