APEX1 Antibody, KO Validated

• Background:

  Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.

• NCBI Gene ID:

  328

• Swiss Prot:

  P27695

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Antibody is Knockout validated.

• Field of Research:

  Transcription

• Purification:

  Affinity purification

• Positive Control:

  HeLa

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 39kDa

• Fragment:

  IgG

• Symbol:

  APEX1

• Positive Control 2:

  HepG2

• NCBI Official Name:

  APEX nuclease (multifunctional DNA repair enzyme) 1

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  APEX1, APE, APX, APE1, APEN, APEX, HAP1, REF1

• Tested Applications:

  WB, IF, IP

• Physical Properties:

  Liquid