PCNA Antibody

• Background:

  The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

• NCBI Gene ID:

  5111

• Swiss Prot:

  P12004

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PCNA (NP_002583.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Signal Transduction, Stem Cell

• Purification:

  Affinity purification

• Positive Control:

  U-251MG

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 36kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:500 - 1:2000IHC: 1:50 - 1:100

• Symbol:

  PCNA

• Positive Control 2:

  A-549

• Positive Control 3:

  HL-60

• Positive Control 4:

  HepG2

• Positive Control 5:

  NIH/3T3

• Positive Control 6:

  SW480

• NCBI Official Name:

  Proliferating cell nuclear antigen

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  PCNA, MGC8367

• Tested Applications:

  WB, IHC

• Physical Properties:

  Liquid