PCNA Antibody

• Background :

  The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

• NCBI Gene ID :

  5111

• Swiss Prot :

  P12004

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PCNA (NP_002583.1) .

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer, Cell Cycle, Signal Transduction, Stem Cell

• Purification :

  Affinity purification

• Positive Control :

  U-251MG

• Concentration :

  Batch dependent

• Buffer :

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight :

  Observed: 36kDa

• Fragment :

  IgG

• Applications Notes :

  WB: 1:500 - 1:2000IHC: 1:50 - 1:100

• Symbol :

  PCNA

• Positive Control 2 :

  A-549

• Positive Control 3 :

  HL-60

• Positive Control 4 :

  HepG2

• Positive Control 5 :

  NIH/3T3

• Positive Control 6 :

  SW480

• NCBI Official Name :

  Proliferating cell nuclear antigen

• NCBI Organism :

  Homo sapiens

• Other Product Names :

  PCNA, MGC8367

• Tested Applications :

  WB, IHC

• Physical Properties :

  Liquid