HLA-DRB3 Antibody

• Background:

  HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB3 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

• NCBI Gene ID:

  3125

• Swiss Prot:

  P79483

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 30-227 of human HLA-DRB3 (NP_072049.2) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  Affinity purification

• Positive Control:

  Raji

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 30kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:500 - 1:2000

• Symbol:

  HLA-DRB3

• Positive Control 2:

  Mouse liver

• Positive Control 3:

  Rat liver

• NCBI Official Name:

  Major histocompatibility complex, class II, DR beta 3

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  DRB3, HLA-DPB1, HLA-DR1B, HLA-DR3B, major histocompatibility complex, class II, DR beta 3, HLA DRB3, HLA class II histocompatibility antigen, DR beta 3 chain, MHC class II HLA-DR beta 3 chain, MHC class II antigen DR beta 3 chain, MHC class II antigen DRB3, human leucocyte antigen DRB3

• Tested Applications:

  WB

• Physical Properties:

  Liquid