POLRMT (CT) Antibody

• Background:

  POLRMT Antibody: This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication. POLRMT mutations impair mitochondrial transcription causing neurological disease. Knock-down of MRPL12 by RNA interference results in instability of POLRMT.

• NCBI Gene ID:

  5442

• Swiss Prot:

  O0411

• Accession Number:

  O0411.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  9561

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  POLRMT Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1203 - A549 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Cow (82%) ; Chimpanzee (100%) ; monkey (82%) ; Cat (90%)

• Buffer:

  POLRMT Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  POLRMT antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human POLRMT has 1 isoform (1230aa, 138.6kD) . Mouse POLRMT has one isoform (1207aa, 136.7kD) . Rat POLRMT has one isoform (1203aa, 135.8kD) . 9561 can detect POLRMT in human, mouse and rat.

• Symbol:

  POLRMT

• Positive Control 2:

  Cat. No. 1224 - Daudi Cell Lysate

• NCBI Official Name:

  RNA polymerase mitochondrial

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Inatomi et al. Biochim Biophys Acta Mol Cell Res. 2022; 1869 (1) ; 119167

• Background Reference 02:

  Olahova et al. Nat Commun. 2021; 12 (1) :1135

• Background Reference 03:

  Nouws et al. J Biol Chem 2016; 291 (2) :989-997

• Other Product Names:

  DNA-directed RNA polymerase, mitochondrial, MtRPOL, POLRMT Isoforms

• Tested Applications:

  ELISA, WB, IF

• Protein ID:

  50403691

• Physical Properties:

  Liquid