CDKN2A Antibody

• Background:

  CDKN2A Antibody: The CDKN2A locus gives rise to 2 distinct transcripts from different promoters. The transcripts have been designated p16 (INK4A) and p14 (ARF) . This chromosomal region undergoes a number of inversions, translocations, heterozygous deletions, and homozygous deletions in a variety of malignant cell lines including those from glioma, non-small cell lung cancer, leukemia, and melanoma. Deletion of the region containing CDKN2A is found in more than half of all melanoma cell lines. Conversely, transfection of CDKN2A suppressed the growth of two independent mesothelioma cell lines, suggesting that inactivation of the CDKN2 gene is an essential step in the etiology of malignant mesotheliomas. CDKN2A induces a G1 cell cycle arrest by inhibiting the phosphorylation of the Rb protein by the cyclin-dependent kinases CDK4 and CDK6. CDKN2A is expressed as at least three distinct isoforms.

• NCBI Gene ID:

  1029

• Swiss Prot:

  P42771

• Accession Number:

  NP_000068

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  8975

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  KO Validation (Figure 1) : Anti-CDKN2A antibodies (8975) specificity was further verified by CDKN2A specific knockout. CDKN2A signal was not detected in CDKN2A knockout 293 cells as compared to that in control wild type cells. KO Validation (8975) Independent Antibody Validation in Cell lines (Figure 2) shows similar CDKN2A expression profile in human cell lines detected by two independent anti-CDKN2A antibodies that recognize different epitopes, 4211 against N-terminus domain and 8975 against the C-terminus domain. CDKN2A proteins are detected only in 293, HeLa and HepG2 cell lines by the two independent antibodies. Independent Antibody Validation in Cell lines4211 8975

• Field of Research:

  Signal Transduction, Homeostasis, Cancer

• Purification:

  CDKN2A Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  CDKN2A Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  CDKN2A antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human CDKN2A has 6 isoforms, including isoform 1 (156aa, 17kD), isoform 2 (105aa, 11kD), isoform 3 (116aa, 12kD), isoform 5 (167aa, 18kD), isoform tumor suppressor ARF (132aa, 14kD) and isoform smARF (85aa, 9kD) . Mouse CDKN2A has 4 isoforms, including isoform 1 (168aa, 18kD), isoform 2 (125aa, 14kD), isoform tumor suppressor ARF (169aa, 19kD) and isoform smARF (125aa, 14kD) . Rat CDKN2A has two isoforms, including isoform 1 (159aa, 17kD) and isoform ARF (160aa, 17kD) . 8975 can detect only human isoforms 1, 2 and 5, but cannot detect mouse and rat.

• Symbol:

  CDKN2A

• Positive Control 2:

  Cat. No. 1211 - HepG2 Cell Lysate

• NCBI Official Name:

  Cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Stone et al. Cancer Res. 1995; 55:2988-94.

• Background Reference 02:

  Kamb et al. Nature Genet. 1994; 8:22-6.

• Background Reference 03:

  Kratzke et al. J. Nat. Cancer Inst. 1995; 87:1870-5.

• Background Reference 04:

  Stott et al. EMBO J. 1998; 17:5001-14.

• Other Product Names:

  CDKN2A Antibody: ARF, MLM, P14, P16, P19, CMM2, INK4, MTS1, TP16, CDK4I, CDKN2, INK4A, MTS-1, P19ARF, P16INK4, P16INK4A, P16-INK4A, Cyclin-dependent kinase 4 inhibitor A

• Tested Applications:

  ELISA, IHC-P, IF, WB

• Protein ID:

  4502749

• Physical Properties:

  Liquid