LAG3 Antibody

• Background:

  The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2) . It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4) . LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5) .

• NCBI Gene ID:

  3902

• Swiss Prot:

  P18627

• Accession Number:

  NP_002277

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  LAG3 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1304 - Human Liver Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat: (80%)

• Buffer:

  LAG3 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  LAG3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  LAG3

• Positive Control 2:

  Cat. No. 11-533 - Human Tonsil Tissue Slide

• NCBI Official Name:

  Lymphocyte activating 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.

• Background Reference 02:

  Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.

• Background Reference 03:

  Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91.

• Background Reference 04:

  Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.

• Other Product Names:

  LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223

• Tested Applications:

  ELISA, WB, IHC, IF

• Protein ID:

  167614500

• Physical Properties:

  Liquid