LSD1 Antibody

• Background:

  Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction (1) . Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2) . LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation (3,4) . Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3) . LSD1-mediated histone demethylation thus may have significant effects on gene expression.

• NCBI Gene ID:

  23028

• Swiss Prot:

  O60341

• Accession Number:

  NP_001009999

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  8223

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  LSD1 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1203 - A549 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  LSD1 antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  LSD1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  At least two isoforms of LSD1 are known to exist; this antibody detects both.

• Isoform:

  Human LSD1 has 2 isoforms, including isoform 1 (852aa, 93kD) and isoform 2 (876aa, 95kD) . Mouse LSD1 has one isoform (853aa, 93kD) and Rat LSD1 also has one isoform (872aa, 95kD) . 8223 can detect LSD1 in human, mouse and rat.

• Symbol:

  KDM1A

• Positive Control 2:

  Cat. No. 10-901 - Human Spleen Tissue Slide

• NCBI Official Name:

  Lysine (K) -specific demethylase 1 A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Shi et al. Cell 2004; 119:941-53.

• Background Reference 02:

  Kouzarides et al. Curr. Opin. Genet. Dev. 2002; 12:198-209.

• Background Reference 03:

  Shi et al. Mol. Cell 2005; 19:857-64.

• Background Reference 04:

  Nakamura et al. Mol. Cell 2002; 10:1119-28.

• Other Product Names:

  Lysine-specific histone demethylase, amine oxidase flavin containing domain protein 2, AOF2

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  58761544

• Physical Properties:

  Liquid