DIRAS3 Antibody

• Background:

  DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1) . Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1) . DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2) . DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3) .

• NCBI Gene ID:

  9077

• Swiss Prot:

  O95661

• Accession Number:

  NP_004666

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Cancer, Autophagy

• Purification:

  DIRAS3 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1313 - Human Testis Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  DIRAS3 antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  DIRAS3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  DIRAS3 antibody is human specific.

• Symbol:

  DIRAS3

• NCBI Official Name:

  DIRAS family, GTP-binding RAS-like 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yu Y, Xu F, Peng H, et al. NOEY2 (ARHI), an imprinted putative tumor suppressor gene in ovarian and breast carcinomas. Proc. Natl. Acad. Sci. USA 1999; 96:214-9.

• Background Reference 02:

  Lu Z, Luo RZ, Lu Y, et al. The tumor suppressor gene ARHI regulates autophagy and tumor dormancy in human ovarian cancer cells. J. Clin. Invest. 2008; 118:3917-29.

• Background Reference 03:

  Klingauf M, Beck M, Berge U, et al. The tumour suppressor DiRas3 interacts with C-RAF and downregulates MEK activity to restrict cell migration. Biol. Cell 2013; 105:91-107.

• Other Product Names:

  DIRAS family GTP-binding RAS-like 3, ARHI, NOEY2

• Tested Applications:

  ELISA, WB

• Protein ID:

  4757772

• Physical Properties:

  Liquid