TRB3 Antibody

• Background :

  TRB3 was initially identified as a novel kinase-like gene that was overexpressed in multiple human tumors and regulated by hypoxia (1) . In the liver, TRB3 binds and inhibits AKT activity, leading to impaired insulin signaling (2), whereas in adipose tissue TRB3 is involved in the regulation of fatty acid oxidation through ubiquitination of Acetyl-CoA carboxylase (3) . Endoplasmic reticulum (ER) stress induces TRB3 expression in skeletal muscle, leading to a decrease in insulin signaling and glucose uptake, suggesting that inhibition of TRB3 expression may be a potential therapeutic treatment for managing insulin resistance (4) .

• NCBI Gene ID :

  57761

• Swiss Prot :

  Q96RU7

• Accession Number :

  NP_066981

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer, Signal Transduction

• Purification :

  TRB3 antibody is affinity chromatography purified via peptide column.

• Positive Control :

  Cat. No. 1405 - Mouse Kidney Tissue Lysate

• Concentration :

  1 mg/mL

• Homology :

  Predicted species reactivity based on immunogen sequence: Bovine: (76%)

• Buffer :

  TRB3 antibody is supplied in PBS containing 0.02% sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  TRB3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment :

  IgG

• Specificity :

  TRB3 antibody is human, mouse and rat reactive. At least two isoforms of TRB3 are known to exist; this antibody will detect both isoforms.

• Symbol :

  TRB3

• NCBI Official Name :

  Tribbles pseudokinase 3

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Bowers AJ, Scully S, and Boylan JF. SKIP3, a novel drosophila tribbles ortholog, is overexpressed in human tumors and is regulated by hypoxia. Oncogene 2003; 22:2823-35.

• Background Reference 02 :

  Du K, Herzig S, Kulkarni RN, et al. TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. Science 2003; 300:1574-7.

• Background Reference 03 :

  Qi L, Heredia JE, Altarejos JY, et al. TRB3 inks the E3 ubiquitin ligase COP1 to lipid metabolism. Science 2006; 312:1763-6.

• Background Reference 04 :

  Koh HJ, Toyoda T, Didesch MM, et al. Tribbles 3 mediates endoplasmic reticulum stress-induced insulin resistance in skeletal muscle. Nat. Commun. 2013; 4:1871.

• Other Product Names :

  Tribbles homolog 3, tribbles pseudokinase 3, TRIB3, NIPK, SINK, SKIP3, C20orf97

• Tested Applications :

  ELISA, WB, IHC-P, IF

• Protein ID :

  31542265

• Physical Properties :

  Liquid