PJA1 Antibody

• Background:

  Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1,2) . Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3) . Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4,5) .

• NCBI Gene ID:

  64219

• Swiss Prot:

  Q8NG27

• Accession Number:

  NP_660095

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Homeostasis

• Purification:

  PJA1 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1303 - Human Brain Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  PJA1 antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PJA1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist; this antibody will detect both isoforms.

• Symbol:

  PJA1

• NCBI Official Name:

  Praja ring finger 1, E3 ubiquitin protein ligase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yu P, Chen Y, Tagle DA, et al. PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain. Genomics 2002; 79:869-74.

• Background Reference 02:

  Zoabi M, Sadeh R, de Bie P, et al. PRAJA1 is a ubiquitin ligase for the polycomb repressive complex 2 proteins. Biochem. Biophys. Res. Commun. 2011; 408:393-8.

• Background Reference 03:

  Doyle JM, Gao J, Wang J, et al. MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases. Mol. Cell 2010; 39:963-74.

• Background Reference 04:

  Saha T, Vardhini D, Tang Y, et al. RING finger-dependent ubiquitination by PRAJA is dependent on TGF-beta and potentially defines the functional status of the tumor suppressor ELF. Oncogene 2006; 25:693-705.

• Other Product Names:

  Praja ring finger 1, E3 ubiquitin-protein ligase Praja-1, PRAJA1, RING finger protein 70, RNF70

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  41281725

• Physical Properties:

  Liquid