LIMA1 Antibody

• Background:

  The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2) . LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3) . Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4) .

• NCBI Gene ID:

  51474

• Swiss Prot:

  Q9UHB6

• Accession Number:

  NP_001107018

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Homeostasis

• Purification:

  LIMA1 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1320 - Human Colon Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (89%), Pig: (72%)

• Buffer:

  LIMA1 antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  LIMA1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist; this antibody will only detect the three largest isoforms.

• Symbol:

  LIMA1

• NCBI Official Name:

  LIM domain and actin binding 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Maul RS and Chang DD. EPLIN, epithelial protein lost in neoplasm. Oncogene 1999; 18:7838-41.

• Background Reference 02:

  Maul RS, Song Y, Amann KJ, et al. EPLIN regulates actin dynamics by cross-linking and stabilizing filaments. J. Cell Biol. 2003; 160:399-407.

• Background Reference 03:

  Abe K and Takeichi M. EPLIN mediates linkage of the cadherin catenin complex to F-actin and stabilizes the circumferential actin belt. Proc. Natl. Acad. Sci. USA 2008; 105:13-9.

• Background Reference 04:

  Zhang S, Wang X, Iqval S, et al. Epidermal growth factor promotes protein degradation of epithelial protein lost in neoplasm (EPLIN), a putative metastasis suppressor, during epithelial-mesenchymal transition. J. Biol. Chem. 2013; 288:1469-79.

• Other Product Names:

  LIM domain and actin-binding protein 1, PP624, EPLIN, SREBP3

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  165905589

• Physical Properties:

  Liquid