LIMA1 Antibody / EPLIN

CAT: 0800-RQ7363Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ7363Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
LIM domain and actin-binding protein 1, also called EPLIN (Epithelial protein lost in neoplasm), is a protein that in humans is encoded by the LIMA1 gene. This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q9UHB6
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human protein (amino acids R9-E744) was used as the immunogen for the LIMA1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the LIMA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This LIMA1 antibody is available for research use only.
Storage Conditions
After reconstitution, the LIMA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the LIMA1 antibody should be determined by the researcher.
Location
Cell membrane, cytoplasm
Image Legend
IHC staining of FFPE human lung squamous cell carcinoma tissue with LIMA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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