TRIP12 Antibody

• Background:

  Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner (1) . TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway and also modulates the NEDD8 pathway (2,3) . TRIP12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site (4) . TRIP12 acts as a key regulator of DNA damage response and the ubiquitin ligase activity of TRIP12 is essential for mouse development (5) .

• NCBI Gene ID:

  9320

• Swiss Prot:

  Q14669

• Accession Number:

  NP_004229

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis, Stem Cell

• Purification:

  TRIP12 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1472 - Rat Colon Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%)

• Buffer:

  TRIP12 antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TRIP12 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  TRIP12 antibody is human, mouse and rat reactive. At least four isoforms are known to exist.

• Symbol:

  TRIP12

• NCBI Official Name:

  Thyroid hormone receptor interactor 12

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lee JW, Choi HS, Gyuris J, et al. Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor. Mol. Endocrinol. 1995; 9:243–54.

• Background Reference 02:

  An CI, Ganio E, and Hagiwara N. Trip12, a HECT domain E3 ubiquitin ligase, targets Sox6 for proteasomal degradation and affects fiber type-specific gene expression in muscle cells. Skelet. Muscle 2013; 3:11.

• Background Reference 03:

  Poulsen EG, Steinhauer C, Lees M, et al. HUWE1 and TRIP12 collaborate in degradation of ubiquitin-fusion proteins and misframed ubiquitin. PLoS One. 2012; 7:e50548.

• Background Reference 04:

  Park Y, Yoon SK, and Yoon JB. The HECT domain of TRIP12 ubiquitinates substrates of the ubiquitin fusion degradation pathway. J. Biol. Chem. 2009; 284:1540-9.

• Other Product Names:

  TRIP12 Antibody: ULF, TRIP-12, KIAA0045, ULF, E3 ubiquitin-protein ligase TRIP12, E3 ubiquitin-protein ligase for Arf

• Tested Applications:

  ELISA, WB, IHC-P, IF, ICC

• Protein ID:

  10863903

• Physical Properties:

  Liquid