TRIM21 Antibody

• Background:

  TRIM21, a member of the tripartite motif (TRIM) family, is a part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus (1) . RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus (2) . TRIM21 also acts as a cytosolic antibody receptor; detection of intracellular antibodies activate immune signaling by stimulating the transcription factor pathways of NF-kappaB, AP-1, IRF3, IRF5, and IRF7, resulting in the production of proinflammatory cytokines and the induction of an antiviral state (3) .

• NCBI Gene ID:

  6737

• Swiss Prot:

  P19474

• Accession Number:

  NP_003132

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  7729

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar TRIM21 expression profile in human cell lines detected by two independent anti-TRIM21 antibodies that recognize different epitopes, 7729 against central domain and 18-413 against recombinant fusion protein. TRIM21 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines7729 18-413

• Field of Research:

  Innate Immunity

• Purification:

  TRIM21 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1302 - Human Lung Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine (73%), Mouse (69%) and Rat (69%) .

• Buffer:

  TRIM21 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TRIM21 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  TRIM21 antibody is human specific. This antibody is predicted to not cross-react with TRIM6.

• Isoform:

  Human TRIM21 has 2 isoforms, including isoform 1 (475aa, 54kD) and isoform 2 (398aa, 45kD) . Mouse TRIM21 has one isoform (470aa, 54kD) and Rat TRIM21 also has one isoform (471aa, 54kD) . 7729 can detect human and possibly mouse and rat.

• Symbol:

  TRIM21

• Positive Control 2:

  Cat. No. 11-801 - Human Small Intestine Tissue Slide

• Positive Control 3:

  Cat. No. 1201 - HeLa Cell Lysate

• Positive Control 4:

  Cat. No. 1220 - SK-N-SH Cell Lysate

• NCBI Official Name:

  Tripartite motif containing 21

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Oke and Wahren-Herlenius. J. Autoimmun. 2012; 39:77-82.

• Background Reference 02:

  Frank et al. Biochem. J. 1995; 305:359-62.

• Background Reference 03:

  McEwan. Nat. Immunol. 14:327-36.

• Other Product Names:

  TRIM21 Antibody: SSA, RO52, SSA1, RNF81, Ro/SSA, E3 ubiquitin-protein ligase TRIM21, 52 kDa Ro protein, SS-A

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  15208660

• Physical Properties:

  Liquid