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TRIM21 Antibody

CAT: 0223-7729-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-7729-01Size:0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
TRIM21, a member of the tripartite motif (TRIM) family, is a part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus (1) . RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus (2) . TRIM21 also acts as a cytosolic antibody receptor; detection of intracellular antibodies activate immune signaling by stimulating the transcription factor pathways of NF-kappaB, AP-1, IRF3, IRF5, and IRF7, resulting in the production of proinflammatory cytokines and the induction of an antiviral state (3) .
NCBI Gene ID
6737
Swiss Prot
P19474
Accession Number
NP_003132
Host
Rabbit
Reactivity
Human
Immunogen
7729
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar TRIM21 expression profile in human cell lines detected by two independent anti-TRIM21 antibodies that recognize different epitopes, 7729 against central domain and 18-413 against recombinant fusion protein. TRIM21 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines7729 18-413
Field of Research
Innate Immunity
Purification
TRIM21 antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1302 - Human Lung Tissue Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Bovine (73%), Mouse (69%) and Rat (69%) .
Buffer
TRIM21 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
TRIM21 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Fragment
IgG
Specificity
TRIM21 antibody is human specific. This antibody is predicted to not cross-react with TRIM6.
Isoform
Human TRIM21 has 2 isoforms, including isoform 1 (475aa, 54kD) and isoform 2 (398aa, 45kD) . Mouse TRIM21 has one isoform (470aa, 54kD) and Rat TRIM21 also has one isoform (471aa, 54kD) . 7729 can detect human and possibly mouse and rat.
Symbol
TRIM21
Positive Control 2
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Positive Control 3
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 4
Cat. No. 1220 - SK-N-SH Cell Lysate
NCBI Official Name
Tripartite motif containing 21
NCBI Organism
Homo sapiens
Background Reference 01
Oke and Wahren-Herlenius. J. Autoimmun. 2012; 39:77-82.
Background Reference 02
Frank et al. Biochem. J. 1995; 305:359-62.
Background Reference 03
McEwan. Nat. Immunol. 14:327-36.
Other Product Names
TRIM21 Antibody: SSA, RO52, SSA1, RNF81, Ro/SSA, E3 ubiquitin-protein ligase TRIM21, 52 kDa Ro protein, SS-A
Tested Applications
ELISA, WB, IHC-P, IF
Protein ID
15208660
Physical Properties
Liquid

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