DAP Antibody

• Background:

  DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1) . DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2) .

• NCBI Gene ID:

  1611

• Swiss Prot:

  P51397

• Accession Number:

  NP_004385

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Autophagy

• Purification:

  DAP Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1308 - Human Small Intestine Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (89%)

• Buffer:

  DAP Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  DAP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  DAP antibody is human, mouse and rat reactive.

• Symbol:

  DAP

• Positive Control 2:

  Cat. No. 11-801 - Human Small Intestine Tissue Slide

• NCBI Official Name:

  Death-associated protein

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Deiss LP, Feinstein E, Berissi H, et al. Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death. Genes Dev. 1995; 9:15-30.

• Background Reference 02:

  Koren I, Reem E, and Kimchi A. DAP1, a novel substrate of mTOR, negatively regulates autophagy. Curr. Biol. 2010; 20:1093-8.

• Other Product Names:

  DAP Antibody: DAP1, Death-associated protein 1, DAP-1

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  4758120

• Physical Properties:

  Liquid