TRIM33 Antibody

• Background :

  TRIM33 Antibody: TRIM24 (TIF1alpha), TRIM28 (TIF1beta), and TRIM33 (TIF1gamma) are three related cofactors belonging to the tripartite motif superfamily that interact with distinct transcription factors (1-2) . The structure of TRIM33 is similar to TRIM24 and TRIM28, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain) (3) . TRIM33 functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells (3) . It interacts with the Smad2/3 transcription factor in hematopoietic, mesenchymal, and epithelial cell types to mediate different transcriptional effects in response to TGF-beta (4) . It acts as an E3 ubiquitin-protein ligase and promotes SMAD4 ubiquitination (5) .

• NCBI Gene ID :

  51592

• Swiss Prot :

  Q9UPN9

• Accession Number :

  NP_056990

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Stem Cell

• Purification :

  TRIM33 Antibody is affinity chromatography purified via peptide column.

• Positive Control :

  Cat. No. 1304 - Human Liver Tissue Lysate

• Concentration :

  1 mg/mL

• Buffer :

  TRIM33 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  TRIM33 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment :

  IgG

• Specificity :

  TRIM33 antibody is human, mouse and rat reactive. At least two isoforms of TRIM33 are known to exist.

• Symbol :

  TRIM33

• Positive Control 2 :

  Cat. No. 10-201 - Human Liver Tissue Slide

• NCBI Official Name :

  Tripartite motif containing 33

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Ransom DG, Bahary N, Niss K, et al. The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis. PLoS Biol. 2004; 2:E237.

• Background Reference 02 :

  Herquel B, Ouararhni K, Khetchoumian K, et al. Transcription cofactors TRIM24, TRIM28, and TRIM33 associate to form regulatory complexes that suppress murine hepatocellular carcinoma. Proc. Natl. Acad. Sci. USA 2011; 108:8212-7.

• Background Reference 03 :

  He W, Dorn DC, Erdjument-Bromage H, et al. Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway. Cell 2006; 125:929-41.

• Background Reference 04 :

  Dupont S, Inui M, Newfeld SJ. Regulation of TGF-β signal transduction by mono- and deubiquitylation of Smads. FEBS Lett. 2012; 586:1913-20.

• Other Product Names :

  TRIM33 Antibody: ECTO, PTC7, RFG7, TF1G, TIF1G, TIFGAMMA, TIF1GAMMA, KIAA1113, E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, Protein Rfg7

• Tested Applications :

  ELISA, WB, IHC-P, IF

• Protein ID :

  74027249

• Physical Properties :

  Liquid