TRIM33 Antibody

• Background:

  TRIM33 Antibody: TRIM24 (TIF1alpha), TRIM28 (TIF1beta), and TRIM33 (TIF1gamma) are three related cofactors belonging to the tripartite motif superfamily that interact with distinct transcription factors (1-2) . The structure of TRIM33 is similar to TRIM24 and TRIM28, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain) (3) . TRIM33 functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells (3) . It interacts with the Smad2/3 transcription factor in hematopoietic, mesenchymal, and epithelial cell types to mediate different transcriptional effects in response to TGF-beta (4) . It acts as an E3 ubiquitin-protein ligase and promotes SMAD4 ubiquitination (5) .

• NCBI Gene ID:

  51592

• Swiss Prot:

  Q9UPN9

• Accession Number:

  NP_056990

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell

• Purification:

  TRIM33 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1304 - Human Liver Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  TRIM33 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TRIM33 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  TRIM33 antibody is human, mouse and rat reactive. At least two isoforms of TRIM33 are known to exist.

• Symbol:

  TRIM33

• Positive Control 2:

  Cat. No. 10-201 - Human Liver Tissue Slide

• NCBI Official Name:

  Tripartite motif containing 33

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ransom DG, Bahary N, Niss K, et al. The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis. PLoS Biol. 2004; 2:E237.

• Background Reference 02:

  Herquel B, Ouararhni K, Khetchoumian K, et al. Transcription cofactors TRIM24, TRIM28, and TRIM33 associate to form regulatory complexes that suppress murine hepatocellular carcinoma. Proc. Natl. Acad. Sci. USA 2011; 108:8212-7.

• Background Reference 03:

  He W, Dorn DC, Erdjument-Bromage H, et al. Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway. Cell 2006; 125:929-41.

• Background Reference 04:

  Dupont S, Inui M, Newfeld SJ. Regulation of TGF-β signal transduction by mono- and deubiquitylation of Smads. FEBS Lett. 2012; 586:1913-20.

• Other Product Names:

  TRIM33 Antibody: ECTO, PTC7, RFG7, TF1G, TIF1G, TIFGAMMA, TIF1GAMMA, KIAA1113, E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, Protein Rfg7

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  74027249

• Physical Properties:

  Liquid