CITED2 Antibody

• Background :

  CITED2 Antibody: CITED2 was initially identified as a potential transcriptional activator with significant homology to MSG1, a melanocyte-specific nuclear protein. CITED2 interacts with the histone acetyltransferase p300/CBP and acts as a coactivator to several DNA-binding transcription factors such as HIF-1 and AP-2. CITED2 also controls proliferation of mouse embryonic fibroblasts via the polycomb group genes Bmi-1 and Mel18 and the tumor suppressor Ink4a/Arf. CITED2 has also been found to be an essential regulator of adult hematopoietic stem cells (HSCs), with conditional deletion of CITED2 in the adult mouse resulting in the loss of HSCs, multilineage bone marrow failure and increased lethality.

• NCBI Gene ID :

  10370

• Swiss Prot :

  Q99967

• Accession Number :

  NP_001161861

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Stem Cell

• Purification :

  CITED2 Antibody is affinity chromatography purified via peptide column.

• Positive Control :

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration :

  1 mg/mL

• Homology :

  Predicted species reactivity based on immunogen sequence: Bovine: (100%)

• Buffer :

  CITED2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  CITED2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  30 kDa

• Fragment :

  IgG

• Specificity :

  Multiple isoforms of CITED2 are known to exist.

• Symbol :

  CITED2

• Positive Control 2 :

  Cat. No. 17-005 - Jurkat Cell Slide

• NCBI Official Name :

  Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Shioda T, Fenner MH, and Isselbacher KJ. MSG1 and its related protein MRG1 share a transcription activating domain. Gene 1997; 204:235-41.

• Background Reference 02 :

  Bhattachatya S, Michels CL, Leung MK, et al. Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1. Genes Dev. 1999; 13:64-75.

• Background Reference 03 :

  Bamforth SD, Braganca J, Eloranta JJ, et al. Cardiac malformations, adrenal agenesis, neural crest defects and exencephaly in mice lacking Cited2, a new Tfap2 co-activator. Nat. Genet. 2001; 29:469-74.

• Background Reference 04 :

  Kranc KR, Bamforth SD, Braganca J, et al. Transcriptional coactivator Cited2 induces Bmi1 and Mel18 and controls fibroblast proliferation via Ink4a/ARF. Mol. Cell. Biol. 2003; 23:7658-66.

• Other Product Names :

  CITED2 Antibody: ASD8, MRG1, VSD2, MRG-1, P35SRJ, MSG-related protein 1

• Tested Applications :

  ELISA, WB, IF

• Protein ID :

  270288756

• Physical Properties :

  Liquid