PLA1A Antibody

• Background:

  PLA1A Antibody: PLA1A is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine (PS) in liposomes and can also hydrolyze PS in apoptotic cells and activate platelets where the resulting 2-acyl-lysophosphatidylserine acts as a lipid mediator for mast cells, T cells, and neural cells, suggesting that a major function of PLA1A may be the production of lysophospholipid mediators. PLA1A is upregulated in rat peripheral blood cells bearing long-term surviving cardiac allograft. PLA1A is also expressed in human THP-1-derived macrophages and this expression is upregulated in cells treated with lipopolysaccharide, a TLR4 ligand. This upregulation is inhibited with corticosteroids, which are often used at high dosages to suppress chronic allograft rejection.

• NCBI Gene ID:

  51365

• Swiss Prot:

  Q53H76

• Accession Number:

  NP_056984

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  PLA1A Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1305 - Human Kidney Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (75%), Bovine: (71%)

• Buffer:

  PLA1A Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PLA1A antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  50 kDa

• Fragment:

  IgG

• Specificity:

  At least three isoforms of PLA1A are known to exist; this antibody will detect all three isoforms.

• Symbol:

  PLA1A

• Positive Control 2:

  Cat. No. 10-401 - Human Kidney Tissue Slide

• NCBI Official Name:

  Phospholipase A1 member A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sato T, Aoki J, Nagai Y, et al. Serine phospholipid-specific phospholipase A that is secreted from activated platelets. A new member of the lipase family. J. Biol. Chem. 1997; 272:2192-8.

• Background Reference 02:

  Aoki J, Nagai Y, Hosono H, et al. Structure and function of phosphatidylserine-specific phospholipase A1. Biochim. Biophys. Acta 2002; 1582:26-32.

• Background Reference 03:

  Hosono H, Homma M, Ogasawa Y, et al. Expression of phosphatidylserine-specific phospholipase A (1) mRNA in human THP-1-derived macrophages. Cell Transplant. 2010; 19:759-64.

• Other Product Names:

  PLA1A Antibody: PSPLA1, PS-PLA1, NMD, PSPLA1, Phospholipase A1 member A, Phosphatidylserine-specific phospholipase A1

• Tested Applications:

  ELISA, WB, IF

• Protein ID:

  7706661

• Physical Properties:

  Liquid