KEAP1 Antibody

• Background:

  KEAP1 Antibody: KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3) -dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) . KEAP1 also targets the down regulation of NF-κ B activity by targeting IKKβ degradation. Mutation of the KEAP1 gene is found in lung cancer.

• NCBI Gene ID:

  9817

• Swiss Prot:

  Q14145

• Accession Number:

  NP_987096

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  7045

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar KEAP1 expression profile in human, mouse (3T3/NIH) and rat (YB2/0) cell lines detected by two independent anti-KEAP1 antibodies that recognize different epitopes, 7045 against C-terminus domain and 22-002 against recombinant fusion protein of human KEAP1. KEAP1 proteins are detected in all the tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines7045 KO Validation (Figure 3) : Anti-KEAP1 antibodies (7045) specificity was further verified by KEAP1 specific knockout. KEAP1 signal was not detected in KEAP1 knockout HeLa cells as compared to that in control wild type cells. KO Validation7045)

• Field of Research:

  Signal Transduction

• Purification:

  KEAP1 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1302 - Human Lung Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Pig: (100%)

• Buffer:

  KEAP1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  KEAP1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least two isoforms of KEAP1 are known to exist.

• Isoform:

  Human KEAP1 has 1 isoform, (624aa, 69.7kD) . Mouse KEAP1 has 1 isoform (624aa, 69.6kD) . Rat KEAP1 has only one isoform (624aa, 69.4 kD) . This antibody detects KEAP1 of human, mouse and rat.

• Symbol:

  KEAP1

• Positive Control 2:

  Cat. No. 10-101 - Human Lung Tissue Slide

• Positive Control 3:

  Cat. No. 1224 - Daudi Cell Lysate

• Positive Control 4:

  Cat. No. 1204– K562 Cell Lysate

• NCBI Official Name:

  Kelch-like ECH-associated protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zhang et al. Mol. Cell. Biol. 2004;24:10941-53.

• Background Reference 02:

  Kobayashi et al. Mol. Cell. Biol. 2004; 24:7130-9.

• Background Reference 03:

  Jiang et al. Int. J. Mol. Med. 2012; 29:946-56.

• Background Reference 04:

  Devling et al. Proc. Natl. Acad. Sci. USA 2005; 102: 7280-5A.

• Other Product Names:

  KEAP1 Antibody: INrf2, KLHL19, INRF2, KIAA0132, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2

• Tested Applications:

  ELISA, WB, IF

• Protein ID:

  45269145

• Physical Properties:

  Liquid