SCUBE3 Antibody

• Background:

  SCUBE3 Antibody: SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart. Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth.. SCUBE3 has been shown to be an endogenous TGF-β receptor ligand and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-β receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-β1, VEGF, Snail, and Slug.

• NCBI Gene ID:

  222663

• Swiss Prot:

  Q8IX30

• Accession Number:

  NP_689966

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  SCUBE3 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1405 - Mouse Kidney Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  SCUBE3 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  SCUBE3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  109 kDa

• Fragment:

  IgG

• Specificity:

  At least two isoforms of SCUBE3 are known to exist. SCUBE3 antibody is predicted to not cross-react with other SCUBE members

• Symbol:

  SCUBE3

• Positive Control 2:

  Cat. No. 10-401 - Human Kidney Tissue Slide

• NCBI Official Name:

  Signal peptide, CUB and EGF-like domain-containing protein 3 precursor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wu BT, Su YH, Tsai MT, et al. A novel secreted, cell-surface glycoprotein containing multiple epidermal growth factor-like repeats and one CUB domain is highly expressed in primary osteoblasts and bones. J. Biol. Chem. 2004; 279:37485-90.

• Background Reference 02:

  Yang RB, Ng CK, Wasserman SM, et al. Identification of a novel family of cell-surface proteins expressed in human vascular endothelium. J. Biol. Chem. 2002; 277:46364-73.

• Background Reference 03:

  Yang HY, Cheng CF, Djoko B, et al. Transgenic overexpression of the secreted, extracellular EGF-CUB domain containing protein SCUBE3 induces cardiac hypertrophy in mice. Cardiovas. Res. 2007; 75:139-47.

• Background Reference 04:

  Wu YY, Peck K, Chang YL, et al. SCUBE3 is an endogenous TGF-beta receptor ligand and regulates the epithelial-mesenchymal transition in lung cancer. Oncogene 2011; 30:3682-93.

• Other Product Names:

  SCUBE3 Antibody: CEGF3, CEGF3, Signal peptide, CUB and EGF-like domain-containing protein 3

• Tested Applications:

  ELISA, WB, IF

• Protein ID:

  31377568

• Physical Properties:

  Liquid