RAB21 Antibody

• Background:

  RAB21 Antibody: RAB21 is a member of a subfamily of small GTP-binding protein genes of the Ras superfamily that plays an important role in intracellular vesicular targeting. In non-polarized Caco-2 cells, RAB21 showed a general endoplasmic reticulum (ER) -like localization, while in polarized cells, RAB21 localized to apical vesicles. RAB21 has been shown to associate with integrin subunits and to be important for receptor entry into cells via RAB5/RAB21 endosomes. The GTPase-activating protein p120RasGAP regulates cell motility by controlling the return of the endocytosed integrins to the plasma membrane by competing with RAB21 for binding to overlapping sites on the α -tail of endocytosed integrin.

• NCBI Gene ID:

  23011

• Swiss Prot:

  Q9UL25

• Accession Number:

  NP_055814

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  RAB21 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1405 - Mouse Kidney Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (95%)

• Buffer:

  RAB21 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  RAB21 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  This antibody is predicted to not cross-react with other Ras-related proteins

• Symbol:

  RAB21

• Positive Control 2:

  Cat. No. 10-401 - Human Kidney Tissue Slide

• NCBI Official Name:

  RAB21, member RAS oncogene family

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Opdam FJ, Kamps G, Croes H, et al. Expression of Rab small GTPases in epithelial Caco-2 cells: Rab21 is an apically located GTP-binding protein in polarised intestinal epithelial cells. Eur. J. Cell Biol. 2000; 79:308-16.

• Background Reference 02:

  Pellinen T and Ivaska J. Integrin traffic. J. Cell Sci. 2006; 119:3723-31.

• Background Reference 04:

  Mai A, Veltel S, Pellinen T, et al. Competitive binding of Rab21 and p120RasGAP to integrins regulates receptor traffic and migration. J. Cell Biol. 2011; 194:291-306.

• Other Product Names:

  RAB21 Antibody: KIAA0118, Ras-related protein Rab-21

• Tested Applications:

  ELISA, WB, IF

• Protein ID:

  7661922

• Physical Properties:

  Liquid