WDR5 Antibody

• Background:

  WDR5 Antibody: WD repeat domain 5 (WDR5) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR5, also known as BIG-3, is expressed in the developing growth plate, accelerates chondrocyte and osteoblast differentiation in vitro, and regulates osteoblast differentiation during embryonic bone development. WDR5 interacts with the pluripotency factor Oct4/POU5F1 and is required for the efficient formation of induced pluripotent stem (iPS) cells.

• NCBI Gene ID:

  11091

• Swiss Prot:

  P61964

• Accession Number:

  NP_060058

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell

• Purification:

  WDR5 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1210 - HEK293 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%), Bovine: (100%)

• Buffer:

  WDR5 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  WDR5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  WDR5 antibody is human specific. WDR5 antibody is predicted to not cross-react with other WDR family members.

• Symbol:

  WDR5

• Positive Control 2:

  Cat. No. 17-010 - HEK293 Cell Slide

• NCBI Official Name:

  WD repeat domain 5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Smith TF, Gaitatzes C, Saxena K, et al. The WD repeat: a common architecture for diverse functions. Trends Biochem. Sci. 1999; 24:181-5.

• Background Reference 02:

  Gori F and Demay MB. BIG-3, a novel WD-40 repeat protein, is expressed in the developing growth plate and accelerates chondrocyte differentiation in vitro. Endocrinology 2004; 145:1050-4.

• Background Reference 03:

  Gori F, Friedman LG, and Demay MB. Wdr5, a WD-40 protein, regulates osteoblast differentiation during embryonic bone development. Dev. Biol. 2006; 295:498-506.

• Background Reference 04:

  Ang YS, Tsai SY, Lee DF, et al. Wdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network. Cell 2011; 145:183-97.

• Other Product Names:

  WDR5 Antibody: SWD3, BIG-3, BIG3, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  16554627

• Physical Properties:

  Liquid