HIG2 Antibody

• Background:

  HIG2 Antibody: HIG1 and HIG2 (Hypoxia-inducible gene 1 and 2, respectively) are known to be induced by hypoxic conditions. HIG2 is induced by hypoxia and by glucose deprivation in cultured cells. In addition, tumor xenografts derived from human cervical cancer cells display increased expression of HIG1 and HIG2 when they are deprived of oxygen. Unlike HIG2, which is ubiquitously expressed and might be an activator and target of the canonical Wnt pathway, the function and the mechanisms underlying its regulation of HIG1 still remained unknown. The putative link between hypoxia and an oncogenic signaling pathway might play an important role in tumorigenesis.

• NCBI Gene ID:

  192286

• Swiss Prot:

  Q9BW72

• Accession Number:

  NP_001092256

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  HIG2 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1203 - A549 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  HIG2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  HIG2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least two isoforms of HIG2 are known to exist; this antibody will detect both isoforms. HIG2 antibody is predicted to not cross-react with HIG1

• Symbol:

  HIGD2A

• NCBI Official Name:

  HIG1 hypoxia inducible domain family, member 2A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Bedo G, Vargas M, Ferreiro MJ, et al. Characterization of hypoxia induced gene 1: expression during rat central nervous system maturation and evidence of antisense RNA expression. Int. J. Dev. Biol. 2005; 49:431-6

• Background Reference 02:

  Simpson JC, Wellenreuther R, Poustka A, et al. Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO Rep. 2000; 1:287-92

• Background Reference 03:

  Denko NC, Schindler C, Koong A, et al. Epigenetic regulation of gene expression in cervical cancer cells by the tumor microenvironment. Clin. Cancer Res. 2000; 6:480-7

• Background Reference 04:

  Gimm T, Wiese M, Teschemacher B, et al. Hypoxia-inducible protein 2 is a novel lipid droplet protein and a specific target gene of hypoxia-inducible factor-1. FASEB J. 2010; 24:4443-58.

• Other Product Names:

  HIG2 Antibody: RCF1b, HIG1 domain family member 2A, mitochondrial, RCF1 homolog B, RCF1b

• Tested Applications:

  ELISA, WB

• Protein ID:

  20270389

• Physical Properties:

  Liquid