MSI2 Antibody

• Background:

  MSI2 Antibody: Musashi2 (MSI2) is an RNA-binding protein that is highly expressed in precursor cells in the ventricular and subventricular zones of the developing mammalian CNS. Like the related MSI1, MSI2 has been suggested to be involved stem cell production and maintenance. MSI2 is the predominant MSI protein in hematopoietic stem cells, and its knockdown leads to reduced engraftment and depletion in vivo. Expression levels of MSI2 are elevated in myeloid leukemia cells lines, and MSI2 appears to cooperate with BCR-ABL1 to induce an aggressive leukemia; the level of MSI2 directly correlates with decreased survival in patients. MSI2 negatively regulates the asymmetric cell fate determinant NUMB, suggesting that this signaling pathway may provide future targets for future therapies.

• NCBI Gene ID:

  124540

• Swiss Prot:

  Q96DH6

• Accession Number:

  EAW94506

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell

• Purification:

  MSI2 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1287 - EL4 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  MSI2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  MSI2 antibody can be stored at 4 ˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  MSI2 antibody is predicted to not cross-react with MSI1. Multiple isoforms of MSI2 are known to exist.

• Symbol:

  MSI2

• Positive Control 2:

  Cat. No. 17-207 - EL4 Cell Slide

• NCBI Official Name:

  Musashi homolog 2 (Drosophila)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sakakibara S, Nakamura Y, Stoh H, et al. RNA-binding protein Musashi2: developmentally regulated expression in neuronal precursor cells and subpopulations of neurons in mammalian CNS. J. Neurosci. 2001; 21:8091-107

• Background Reference 02:

  Kharas MG, Lengner CJ, Al-Shahrour F, et al. Musashi-2 regulates normal hematopoiesis and promotes aggressive myeloid leukemia. Nat. Med. 2010; 16:903-8

• Background Reference 03:

  Ito T, Kwon HY, Zimdahl B, et al. Regulation of myeloid leukaemia by the cell-fate determinant Musashi. Nature 2010; 466:765-8.

• Background Reference 04:

  Griner LN and Reuther GW. Aggressive myeloid leukemia formation is directed by the Musashi2/Numb pathway. Cancer Biol. Ther. 2010; 10:979-82

• Other Product Names:

  MSI2 Antibody: MSI2H, RNA-binding protein Musashi homolog 2, Musashi-2

• Tested Applications:

  ELISA, WB, ICC, IHC-P, IF

• Protein ID:

  20373175

• Physical Properties:

  Liquid