ZFP219 Antibody

• Background:

  ZFP219 Antibody: ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.

• NCBI Gene ID:

  51222

• Swiss Prot:

  Q9P2Y4

• Accession Number:

  NP_001095142

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell

• Purification:

  ZFP219 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1287 - EL4 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  ZFP219 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  ZFP219 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least two isoforms of ZFP219 are known to exist; this antibody will recognize both.

• Symbol:

  ZNF219

• NCBI Official Name:

  Zinc finger protein 219

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sakai T, Toyoda A, Hashimoto K, et al. Isolation and characterization of a novel zinc finger gene, ZNF219, and mapping to the human chromosome 14q11 region. DNA Res. 2000; 7:137-41.

• Background Reference 02:

  Sakai T, Hino K, Wada S, et al. Identification of the DNA binding specificity of the human ZNF219 protein and its function as a transcriptional repressor. DNA Res. 2003; 10:155-65.

• Background Reference 03:

  Takigawa Y, Hata K, Muramatsu S, et al. The transcription factor Znf219 regulates chondrocyte differentiation by assembling a transcription factory with Sox9. J. Cell Sci. 2010; 123:3780-8.

• Other Product Names:

  ZFP219 Antibody: ZFP219, Zinc finger protein 219

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  156415996

• Physical Properties:

  Liquid